TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates

Abstract Titanium dioxide nanotubes (TiO2-nts) were incorporated into a glass ionomer cement (GIC) with improved mechanical properties and antibacterial activity. The aims of the present in vitro study were to define the elemental characterization, aluminum (Al) release rate, and initial working time for GIC reinforced with TiO2-nts, in an experimental caries model. TiO2-nts were incorporated into GIC powder components at 5% by weight, and compared with unblended GIC. Experimental approaches used energy-dispersive spectrometry (EDS), atomic absorption spectrophotometry (AAS), and brightness loss to define surface element properties, Al release rates, and initial working time, respectively. Statistical analysis was performed by 2-way ANOVA, Tukey's test, generalized linear models, and Student's t test (a = 0.05). EDS data analysis revealed that TiO2-nts incorporated into GIC had no significant impact on the typical elemental composition of GICs in an in vitro caries model. Regarding the demineralizing solution, GIC with TiO2-nt significantly decreased the Al release rate, compared with the control group (p < 0.0001). Moreover, TiO2-nt incorporated into GIC did not alter the initial working time of the material (p > 0.05). These findings add information to our scientific body of knowledge concerning the potential impact of TiO2-nt on the performance of conventional GICs.

CHX stabilizes the resin/demineralized dentin interface

Abstract The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on microtensile bond strength (µTBS) between an adhesive system and under 3 dentin conditions. For that, this study evaluated the adhesive interface at initial, after 6 months and 1 year of storage. Forty-eight human third molars were prepared and randomly divided into 3 groups, according to dentin substrates: sound dentin (Sd), caries-infected dentin (Ci) and caries-affected dentin (Ca). The groups were subdivided into two according to the dentin pre-treatment: application of 2% CHX or without pre-treatment (control). The dentin surfaces were etched with 35% phosphoric acid gel and bonded with Adper Single Bond 2 (3M ESPE) adhesive system according to manufacturer's instructions. Subsequently, the specimens were stored in deionized water at 37°C for 24h, 6 months and 1 year. Two additional teeth were used to analyze the bonding interfaces by SEM. Data was submitted to three-way ANOVA in a split plot design and Tukey's test (α = 0.05). The results showed that Ci decreased µTBS values when compared to Ca and Sd, regardless storages time or treatment. Stored samples for 6 months and 1 year decreased the µTBS for the control group, but no difference was found between storages time for the CHX group. As a conclusion, the 2% CHX application after etching showed improved dentin bond strength in the storage time, regardless of the substrates evaluated.

Resumo O objetivo deste estudo foi avaliar o efeito do digluconato de clorexidina 2% (CHX) na resistência de união à microtração (µTBS) entre um sistema adesivo e sob 3 condições de dentina. Para tanto, este estudo avaliou a interface adesiva no início, após 6 meses e 1 ano de armazenamento. Quarenta e oito terceiros molares humanos foram preparados e divididos aleatoriamente em 3 grupos, de acordo com os substratos dentinários: dentina hígida (Dh), dentina infectada por cárie (Di) e dentina afetada por cárie (Da). Os grupos foram subdivididos em dois de acordo com o pré-tratamento: aplicação de CHX 2% ou sem pré-tratamento (controle). As superfícies da dentina foram condicionadas com gel de ácido fosfórico a 35%, em seguida, utilizou-se o sistema adesivo Adper Single Bond 2 (3M ESPE) de acordo com as instruções do fabricante. Posteriormente, os corpos-de-prova foram armazenados em água deionizada a 37°C por 24h, 6 meses e 1 ano. Dois dentes adicionais foram usados ​​para analisar as interfaces de união no MEV. Os dados foram submetidos a ANOVA três fatores em esquema de parcela subdividida e ao teste de Tukey (α = 0,05). Os resultados mostram que a Di diminuiu os valores de µTBS quando comparado as Da e Dh, independente do tempo de armazenamento ou tratamento. Amostras armazenadas por 6 meses e 1 ano diminuíram a µTBS para o grupo de controle, mas nenhuma diferença foi encontrada entre o tempo de armazenamento para o grupo CHX. A aplicação de CHX 2% após o condicionamento apresentou melhora na resistência de união à dentina no tempo de armazenamento, independente dos substratos avaliados.

Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence

Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey's and Dunn's post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.

Can composition and preheating improve infiltrant characteristics and penetrability in demineralized enamel?

Abstract The composition of infiltrants can influence their physical properties, viscosity and depth of penetration (DP). Strategies are used to increase the DP, such as the addition of diluents or the use of heat. This study aimed to evaluate the effect of preheating and composition on physical properties and DP of infiltrants in demineralized enamel. The groups were assigned, and the following experimental formulations were made: 25%BisEMA +75%TEGDMA; 25%BisEMA +65%TEGDMA +10%ethanol; 25%BisEMA +65%TEGDMA +10%HEMA; 100%TEGDMA; 90%TEGDMA +10%ethanol; 90%TEGDMA +10%HEMA. The samples were photoactivated at two temperatures (25°C and 55°C). Degree of conversion (DC) was performed using an infrared spectrophotometer. Elastic modulus (E), flexural strength (FS) and contact angle (CA) tests were also performed. The DP of an infiltrant in demineralized enamel was determined by confocal laser scanning microscopy (CLSM) using an indirect labeling technique. The data were analyzed by two-way ANOVA and Tukey's test. DC increased after preheating in all the groups; however, 90%TEGDMA+10%ethanol showed the lowest DC for both temperatures, and the lowest E. Preheating did not influence E or FS. The CA increased at 55°C for most groups, but decreased for groups containing HEMA. Temperature did not seem to influence DP, and Icon showed the lowest DP values. The 100%TEGDMA composition showed more homogeneous penetration, whereas Icon showed heterogeneous and superficial penetration. The preheating technique does not improve all properties in all the material compositions. The composition of a material can influence and improve its properties.

Color stability and gloss of esthetic restorative materials after chemical challenges

Abstract This study evaluated gloss and color changes of esthetic restorative materials subjected to different acidic beverages. Specimens of resin composites (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD)) were prepared and the initial surface gloss and color (ΔE) were measured (n=10). Then, the specimens were immersed in 4 mL of each of the different beverages (cranberry juice; Coca-Cola; coffee or artificial saliva) during 15 min, 3x/day for 14 days and new gloss and color readings were obtained. Color change was evaluated with the ΔE formula and gloss change values were obtained by the formula: (final gloss - initial gloss). Data was submitted to two-way ANOVA followed by Tukey's post hoc test (a=0.05). CD showed the lowest color change among resin composites. The highest ΔE values were obtained after immersion in coffee and cranberry juice. Coffee promoted the highest gloss change (worst gloss retention), followed by cranberry juice, Coca-cola and artificial saliva (p<0.05). The type of beverage significantly influenced the gloss of resin composites. Coca-cola reduced gloss of the three resin composites in a similar manner. Coffee affected the ED gloss more than that of Z350 and CD, while cranberry juice affected Z350 more than ED and CD. Saliva had a more pronounced effect on the gloss retention of CD than ED. The beverages used in this study influenced the optical surface properties of the composites studied.

Resumo O objetivo foi avaliar as alterações de brilho e cor de materiais restauradores estéticos submetidos ao armazenamento em diferentes bebidas ácidas. Foram preparados espécimes de compósitos de resina (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD) e o brilho e a cor (ΔE) de superfície mensurados (n=10). Em seguida, os espécimes foram imersos em 4 mL de cada uma das diferentes bebidas (suco cranberry, Coca-Cola, café, ou saliva artificial) durante 15 min, 3x/dia durante 14 dias e o brilho e a cor novamente mensurados. A alteração de cor foi avaliada pela fórmula ΔE e os valores de alteração de brilho foram obtidos pela fórmula: (brilho final - brilho inicial). Os dados foram submetidos à ANOVA e Tukey (a = 0,05). CD apresentou menor alteração de cor quando comparado aos demais compósitos e maiores valores de ΔE foram obtidos após imersão em suco de café e cranberry. O café promoveu maior perda de brilho, seguido pelo suco cranberry, Coca-cola e saliva artificial (p <0,05). O tipo de bebida influenciou significativamente no brilho dos compósitos resinosos. Coca-cola reduziu o brilho dos três compósitos de maneira similar. O café promoveu maior alteração do brilho do ED, quando comparado ao Z350 e CD, enquanto o suco de cranberry afetou mais o Z350 do que o ED e o CD. A saliva teve efeito mais significativo na perda do brilho do CD do que o ED. As bebidas utilizadas neste estudo influenciaram as propriedades ópticas de superfície dos compósitos.

Antimicrobial activity of mouth rinses against bacteria that initially colonizes dental's surface / Atividade antimicrobiana de enxaguatórios bucais sobre bactérias que iniciam a colonização das superfícies dentais

Abstract Introduction Much advertising in mouthwash is conveyed in all media appealing to the anti-plaque effect and rendering a disservice to the community. Mouth rinses are available over-the-count and differ on their compositions and antimicrobial effectiveness. Objective In this study, we evaluated the antimicrobial activity of 35 widely available mouth rinses against bacterial species involved in initiation of dental biofilm - Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, and Streptococcus sanguinis. Material and method The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the evaluated mouth rinses were determined according to the Clinical & Laboratory Standards Institute protocols. Data were submitted to Kruskal-Wallis test and Mann-Whitney post hoc (α=0.05). Result About 70% of the mouth rinses achieved high antibacterial activity and 30%, a low antibacterial activity against all the species tested. The most ineffective mouth rinse showed antibacterial activity (MIC) at 1:1 dilution, while the most effective showed activity even at 1:2048 dilution, which may imply prolonged effect in the mouth. About 51% of mouth rinses showed bactericidal activity, and it was verified that cetylpyridinium chloride or chlorhexidine digluconate containing in the formulation were associated with the highest activity. Conclusion Most - but not all - mouth rinses commercially available are effective in inhibiting in vitro initial colonizers of dental surfaces.

Resumo Introdução Muita publicidade sobre enxaguatórios bucais é veiculada em todos os meios de comunicação apelando para o efeito anti-placa e prestando um desserviço à comunidade. Grande quantidade de enxaguatórios bucais está disponível no mercado e estes diferem em suas composições e eficácia antimicrobiana. Objetivo Neste estudo, avaliamos a atividade antimicrobiana de 35 enxaguatórios bucais amplamente disponíveis contra espécies bacterianas envolvidas na iniciação do biofilme dental - Streptococcus gordonii, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius e Streptococcus sanguinis. Material e método A Concentração Inibitória Mínima (CIM) e a Concentração Bactericida Mínima (CBM) dos enxaguatórios avaliados foram determinadas de acordo com os protocolos do Clinical & Laboratory Standards Institute. Os dados foram submetidos ao teste Kruskal-Wallis e Mann-Whitney post hoc (α=0,05). Resultado Aproximadamente 70% dos enxaguatórios bucais alcançaram alta atividade antibacteriana e 30%, baixa atividade antibacteriana contra todas as espécies testadas. O enxaguatório bucal mais ineficaz mostrou atividade antibacteriana (CIM) na diluição de 1:1, enquanto a mais eficaz mostrou atividade mesmo na diluição de 1:2048, o que pode implicar em efeito prolongado na boca. Cerca de 51% dos enxaguatórios bucais apresentaram atividade bactericida, e verificou-se que formulações contendo cloreto de cetilpiridíneo ou digluconato de clorexidina estavam associados à maior atividade. Conclusão A maior parte - mas não todos - dos enxaguatórios bucais comercialmente disponíveis são eficazes na inibição de colonizadores iniciais de superfícies dentárias in vitro.

Doxycycline-containing glass ionomer cement for arresting residual caries: an in vitro study and a pilot trial

Abstract In a previous study, we demonstrated that the incorporation of doxycycline hyclate (DOX) into resin-modified glass ionomer cement (RMGIC) inhibited important cariogenic microorganisms, without modifying its biological and mechanical characteristics. In this study, we keep focused on the effect of that experimental material as a potential therapy for arresting residual caries by analyzing other in vitro properties and conducting a pilot clinical trial assessing the in vivo effect of DOX-containing RMGIC on residual mutans streptococci after partial carious removal in primary molars. Specimens of the groups RMGIC (control); RMGIC + 1.5% DOX; RMGIC + 3% DOX; and RMGIC + 4.5% DOX were made to evaluate the effect of DOX incorporation on surface microhardness and fluoride release of RMGIC and against biofilm of Streptococcus mutans. Clinical intervention consisted of partial caries removal comparing RMGIC and RMGIC + 4.5% DOX as lining materials. After 3 months, clinical and microbiologic evaluations were performed. Data were submitted to ANOVA/Tukey or Wilcoxon/Mann-Whitney set as α=0.05. Fluoride release and surface microhardness was not influenced by the incorporation of DOX (p>0.05). There was a significant reduction of S. mutans biofilm over the material surface with the increase of DOX concentration. After clinical trial, the remaining dentin was hard and dry. Additionally, mutans streptococci were completely eliminated after 3 months of treatment with RMGIC + 4.5% DOX. The incorporation of DOX provided better antibiofilm effect, without jeopardizing fluoride release and surface microhardness of RMGIC. This combination also improved the in vivo shortterm microbiological effect of RMGIC after partial caries removal.

Biological and mechanical degradation affecting the surface properties of aesthetic restorative

To evaluate the roughness (Ra), Knoop hardness (KHN) and change of color (ΔE) of esthetic restorative materials (Filtek Z350-composite nanoparticle; Empress Direct-composite nanohybrid and IPS e.Max-ceramic) subjected to contact with the Streptococcus mutans biofilm (biological degradation) associated with abrasion generated by tooth brushing (mechanical degradation). Methods: Ten specimens of each material were prepared, and the surface properties initial were evaluated. All specimens were exposed to Streptococcus mutans inoculum; after 7 days, surface properties were evaluated. The specimens were submitted to a 30,000 toothbrushing cycles, using a toothpaste slurry, then, surface properties were evaluated again. Data were analyzed by Proc-Mixed, One-way ANOVA, Tukey-Kramer and Tukey's tests (α = 0.05). Results: At the baseline, ceramic showed the highest Ra and KHN values; after the biological degradation the composites showed increased Ra, but KHN did not change; after the mechanical degradation, Empress showed decreased Ra and Z350 showed similar Ra, the KHN increased to both composites, and all materials had increased lightness after the mechanical degradation. Conclusions: The results suggest that, when exposed to Streptococcus mutans biofilm and toothbrush abrasion, the ceramics undergoes minimal degradation and the composites exhibited variable degradation, depending on the composition of the material (AU)

Influence of Different Concentration and Ratio of a Photoinitiator System on the Properties of Experimental Resin Composites

Abstract The aim in this study was to evaluate the influence of different ratio of camphorquinone/tertiary amine concentration on the flexural strength (FS), elastic modulus (EM), degree of conversion (DC), yellowing (YL), water sorption (WS) and water solubility (WSL) of experimental composites. Thus, acrylate blends were prepared with different camphorquinone (CQ) and amine (DABE) concentrations and ratios by weight: (CQ/DABE%): 0.4/0.4% (C1), 0.4/0.8% (C2), 0.6/0.6% (C3), 0.6/1.2% (C4), 0.8/0.8% (C5), 0.8/1.6% (C6), 1.0/1.0% (C7), 1.0/2.0% (C8), 1.5/1.5% (C9), 1.5/3.0% (C10). For the FS and EM, rectangular specimens (7x2x1 mm, n=10) were photo-activated by single-peak LED for 20 s and tested at Instron (0.5 mm/min). Then, the same specimens were evaluated by FTIR for DC measurement. For YL, disks (5x2 mm, n=10) were prepared, light-cured for 20 s and evaluated in spectrophotometer using the b aspect of the CIEL*a*b* system. For WS and WSL, the volume of the samples was calculated (mm³). For WS and WSL, composites disks (5x0.5 mm, n=5) were prepared. After desiccation, the specimens were stored in distilled water for 7 days and again desiccated, in order to measure the WS and WSL. Data were submitted to one-way ANOVA and Tukey's test (5%). The groups C8, C9 and C10 showed higher DC, EM and YL means, compared to other composites. Therefore, the FS and WS values were similar among all groups. Also, C1, C2 and C3 presented higher WSL in 7 days, compared to other composites. In general, higher concentrations of camphorquinone promoted higher physical-mechanical properties; however, inducing higher yellowing effect for the experimental composites

Resumo O objetivo foi avaliar a influência da concentração e proporção de canforoquinona (CQ)/amina terciária (DABE) na resistência à flexão (RF), módulo flexural (MF), grau de conversão (GC), amarelamento (AM), sorpção (SA) e solubilidade em água (SL) de compósitos experimentais. Blendas acrilatas foram preparadas com diferentes concentrações e proporções de CQ/DABE em peso, como segue: (CQ/DABE%): 0,4/0,4% (C1); 0,4/0,8% (C2); 0,6/0,6% (C3); 0,6/1,2% (C4); 0,8/0,8% (C5); 0,8/1,6% (C6); 1,0/1,0% (C7); 1,0/2,0% (C8); 1,5/1,5% (C9); 1,5/3,0% (C10). Para RF e MF, espécimes retangulares (7x2x1 mm, n=10) foram fotoativados com LED de pico único (Radii Cal) por 20 s e testados em máquina Instron (0,5 mm/min). Após, o GC dos mesmos espécimes (fragmentos) foi mensurado por FTIR. Para AM, discos de compósito (5x2 mm, n=10) foram preparados, fotoativados por 20 s e imediatamente avaliados em espectrofotômetro, considerando o aspecto b do sistema CIEL*a*b*. Para SA e SL, discos de compósito (5x0,5 mm, n=5) foram preparados e seus volumes calculados (mm³). Após desidratação, as amostras foram pesadas e armazenadas em água destilada por 7 dias, pesadas e novamente desidratadas e pesadas, para se calcular o SA e SL. Os dados foram submetidos a one-way ANOVA e teste de Tukey (5%). Os grupos C8, C9 e C10 mostraram maior GC, MF e AM, comparado aos outros grupos. No entanto, RF e SA foram similares entre todos os grupos. Ainda, C1, C2 e C3 apresentaram maior SL em 7 dias comparado aos outros grupos. Em geral, maiores concentrações de CQ promoveram melhores propriedades físico-mecânicas; no entanto, levaram ao maior amarelamento dos compósitos.

Influence of Different Dentin Substrate (Caries-Affected, Caries-Infected, Sound) on Long-Term muTBS

Abstract The aim of this study was to evaluate the μTBS in different dentin substrates and water-storage periods. Twenty-four dentin blocks obtained from sound third molars were randomly divided into 3 groups: Sound dentin (Sd), Caries-affected dentin (Ca) and Caries-infected dentin (Ci). Dentin blocks from Ca and Ci groups were subjected to artificial caries development (S. mutans biofilm). The softest carious tissue was removed using spherical drills under visual inspection with Caries Detector solution (Ca group). It was considered as Ci (softer and deeply red stained dentin) and Ca (harder and slightly red stained dentin). The Adper Single Bond 2 adhesive system was applied and Z350 composite blocks were built in all groups. Teeth were stored in deionized water for 24 h at 37 ºC and sectioned into beams (1.0 mm2 section area). The beams from each tooth were randomly divided into three storages periods: 24 h, 6 months or 1 year. Specimens were submitted to µTBS using EZ test machine at a crosshead speed of 1.0 mm/min. Failure mode was examined by SEM. Data from µTBS were submitted to split plot two-way ANOVA and Tukey’s HSD tests (a=0.05). The µTBS (MPa) of Sd (41.2) was significantly higher than Ca (32.4) and Ci (27.2), regardless of storage. Ca and Ci after 6 months and 1 year, presented similar µTBS. Mixed and adhesive failures predominated in all groups. The highest µTBS values (48.1±9.1) were found for Sd at 24 h storage. Storage of specimens decreased the µTBS values for all conditions.

Resumo O objetivo neste estudo foi avaliar a resistência de união à microtração (RUµT) de um sistema adesivo convencional (Adper Single Bond 2 - SB) em diferentes substratos dentinários e períodos de armazenagem. Vinte e quatro blocos de dentina foram obtidos de terceiros molares hígidos e separados aleatoriamente em 3 grupos (n=8): dentina sadia (Ds), dentina afetada (Da) e dentina infectada (Di). A Da e a Di foram submetidas ao desenvolvimento biológico artificial de cárie (S. mutans). O tecido cariado amolecido foi removido usando broca esférica sob inspeção visual com a solução Caries Detector (grupo Da). Considerou-se como Di a dentina amolecida e fortemente pigmentada de vermelho e como Da, a dentina hígida e levemente pigmentada de vermelho. O sistema adesivo SB foi aplicado de acordo com as recomendações do fabricante e blocos da resina composta Z350 foram construídos (6 mm de altura). O conjunto (dente/bloco de resina) foi armazenado em água deionizada por 24 horas a 37 °C. Estes foram seccionados em palitos (1,0 mm2 de área), que foram separados aleatoriamente em 3 períodos de armazenagem: 24 horas, 6 meses e 1 ano. Os palitos foram submetidos ao ensaio de resistência de união à microtração na máquina EZ teste a uma velocidade de 1,0 mm/min. Dados de RUµT foram submetidos à Análise de Variância 2 fatores em esquema de parcela subdividida e ao teste de Tukey (a=0,05). Os valores de resistência (MPa) da Ds (41,2) foram significativamente maiores do que os da Da (32,4) e Di (27,2), independente do tempo de armazenagem. Di e Da, 6 meses e 1ano, apresentaram valores similares de resistência de união. As falhas adesivas e mistas foram predominantes para todos os grupos. Em conclusão, os maiores valores de RUµT (48,1±9,1) foram verificados para a Ds e 24 h de armazenagem. A armazenagem diminuiu os valores de RUµT para todas as condições.

Molecular and morphological surface analysis: effect of filling pastes and cleaning agents on root dentin

Abstract The quality of the dentin root is the most important factor for restoration resin sealing and drives the outcome of endodontic treatment. Objective This study evaluated the effect of different filling pastes and cleaning agents on the root dentin of primary teeth using Fourier-transformed Raman spectroscopy (FT-Raman), micro energy-dispersive X-ray fluorescence (µ-EDXRF) and scanning electron microscopic (SEM) analysis. Material and Methods Eighty roots of primary teeth were endodontically prepared and distributed into 4 groups and filled according to the following filling pastes: Control-no filling (CP), Calen®+zinc oxide (CZ), Calcipex II® (CII), Vitapex® (V). After seven days, filling paste groups were distributed to 4 subgroups according to cleaning agents (n=5): Control-no cleaning (C), Ethanol (E), Tergenform® (T), 35% Phosphoric acid (PA). Then, the roots were sectioned and the dentin root sections were internally evaluated by FT-Raman, µ-EDXRF and SEM. Data was submitted to two-way ANOVA and Tukey tests (α=0.05). Results Regarding filling pastes, there was no significant difference in organic content. CP provided the lowest calcium values and, calcium/phosphoric ratio (Ca/P), and the highest phosphoric values. For cleaning agents there was no difference in organic content when compared to the C; however, T showed significantly higher calcium and Ca/P than PA. All groups showed similar results for phosphorus. The dentin smear layer was present after use of the cleaning agents, except PA. Conclusion The filling pastes changed the inorganic content, however they did not change the organic content. Cleaning agents did not alter the inorganic and organic content. PA cleaned and opened dentin tubules.

Abordagem integral em odontopediatria: relato de caso clínico / Integral Approach in Pediatric Dentistry: clinical case report

Objetivo: Relatar um caso clínico envolvendo a abordagem cirúrgica/restauradora e medidas preventivas. Relato do caso: Paciente MEMO, 6 anos e 8 meses de idade, feminino, com alto risco/atividade de cárie, apresentando biofilme visível em superfícies lisas livres, lesões de cárie ativa, perda precoce do dente 85, fístula relacionada com a região do dente 75, mudança de cor do dente 61 e perda significativa de dimensão vertical posterior, apresentou-se para tratamento na Clínica de pós-graduação em Odontopediatria da FOP/Unicamp. O plano de tratamento consistiu na abordagem integral focada na promoção da saúde como desfecho principal, ou seja, mudança de comportamento em relação à saúde bucal, e como desfecho secundário, a abordagem cirúrgica/restauradora. Instrução de higiene bucal foi realizada em todas as sessões. O manejo do comportamento foi conduzido por meio da evidenciação e controle de biofilme, profilaxia e adequação do meio bucal seguido da extração do dente 75, selamento de fossas e fissuras dos dentes 36, 46 e 16 e restaurações no 55 e 65. Mantenedor de espaço removível inferior foi realizado. Conclusões: Após seis meses pôde-se observar ausência de biofilme visível, o índice de placa estava controlado e não havia evidência de novas lesões de cárie. Pode-se concluir que o sucesso obtido neste caso destaca a importância da abordagem integral focada na promoção da saúde na clínica odontopediátrica.

Objective: To report a clinic case involving the surgical/restorative approach and preventive actions. Case report: The patient MEMO, aged 6y and 8mo, female, with high caries risk/activity, presenting visible biofilm on free smooth surfaces, active carious lesions, early loss of the 85 tooth, fistula related with the 75 tooth region, color change on 61 tooth and significant loss of posterior vertical dimension has been driven to Pediatric Dentistry Graduate Clinic at FOP/ Unicamp. Treatment plan consisted on integral approach focused as primary outcome on health promotion, behavior change in relation to oral health, and as secondary outcome, the surgical/ restorative approach. Oral hygiene instruction was conducted in all sessions. The behavior management was conducted by evidence and control of visible dental plaque, prophylaxis and oral environment adequation; extraction of 75 tooth, pit and fissure sealing of the 36, 46, 16 teeth, and restorations in the 55 and 65. Removable lower space maintainer appliance was made. Conclusions: After 6 months it could be observed that there was not visible biofilm, the IP was controlled. In addition, there was no evidence of new caries lesions. It could be concluded that the success obtained on this clinical case bring about the importance of the integral performance focused on healthy promotion in pediatric dentist clinic.

Polishing techniques effect on microhybrid, nanohybrid and nanofilled composites color and surface roughness stability / Efeito de técnicas de polimento na estabilidade da cor e rugosidade superficial de resinas compostas microhibridas nanohibridas e nanoparticuladas

Resin roughness and staining is a common problem in dentistry. Objective: the aim of this study was to verify the influence of single and multiple-steps polishing techniques on color stability and surface roughness of differents composite resins. Groups were (n=10): FS+CS. Filtek Supreme XT + clear strip; FS+PG. Filtek Supreme XT+PoGo; FS+SF. Filtek Supreme XT+Sof-Lex; AM+CS. Amelogen+clear strip; AM+PG. Amelogen+PoGo; AM+SF. Amelogen+Sof-Lex; TEC+CL .TetricEvoCeram + clear strip; TEC+PG .Tetric EvoCeram+ PoGo; TEC+SF .Tetric EvoCeram+Sof-Lex; CED+CS .Ceram X Duo+clear strip; CED+PG. Ceram X Duo+PoGo; CED+SF. Ceram X Duo+Sof- Lex. The initial color and roughness were determined before and after the polishing procedures. All specimens were stored for 7 days in coffee solution and the color assessment was performed. Two specimens from each group were evaluated by scanning electron microscopy. Roughness and color data were submitted to ANOVA and Tukey tests(p<0.05). All tested materials presented color alterations after coffee solution storage; the highest E values were observed for clear strips and all composite resins, as following: G4 (9h)>G7(5.8f)=G10(5.7ef)>G1(3.7c), followed by the G6(5.9f)>G9(3.1bc)=G12(2.1ab)>G3(1.7a). G3, after 7 days, presented the lowest values for E(1.704). The lowest roughness values were observed when the clear strips were used regardless material type. The Sof-Lex system provided the lowest surface roughness values for all tested composite resins. Among composite resins the highest roughness values were found for Amelogen (G5­0.49 and G6­0.35), regardless polishing technique. All tested materials presented changes in color after immersion in coffee solution. The roughness surface and the staining of composite resins are material and polishing technique dependents.

Rugosidade e pigmentação de resinas são problemas comuns em odontologia. Objetivo: Avaliar influência das técnicas de um e múltiplos-passos de polimento na estabilidade de cor e rugosidade de resinas. Os grupos (n=10): G1.Filtek Supremo XT+Tira poliéster; G2.Filtek SupremeXT+PoGo; G3.Filtek Supreme XT+ Sof-Lex; G4.Amelogen+Tira poliéster; G5.Amelogen + PoGo; G6.Amelogen + Sof-Lex; G7.Tetric EvoCeram +Tira poliéster; G8.Tetric EvoCeram+PoGo; G9.Tetric EvoCeram+Sof-Lex; G10.CeramXDuo+Tira poliéster; G11.Ceram X Duo+PoGo; G12.Ceram X Duo+Sof-Lex. A Cor inicial e rugosidade foram determinadas antes e apóso polimento. Após, foram armazenadas durante 7 dias em solução de café e nova avaliação da cor foi realizada. Dois espécimes de cada grupo foram avaliados pela microscopia eletrônica de varredura. Dados de rugosidade e cor foram submetidos à ANOVA e teste Tukey (p <0,05). Todas as resinas testadas apresentaram alterações de cor após armazenamento em solução de café; foram observados os maiores valores de AE em tiras de poliésterpara as resinas,a seguir: G4 (9h)>G7 (5,8f) = G10 (5,7ef)>G1 (3,7c), seguindo-se a G6 (5,9f)>9 (3,1bc)=G12 (2,1ab)>G3 (1,7a). G3, após 7 dias, apresentaram os menores valores para AE (1,704). Menores valores de rugosidade foram observados para as tiras de poliéster, independentemente do material. Menores valores de rugosidade foram encontrados no sistema Sof-Lex. Entre as resinas valores maiores de rugosidade foram encontrados para Amelogen (G5-0,49 e G6-0,35), para as duas técnicas. Todos os materiais testados apresentaram alterações na cor após imersão em solução de café. A rugosidade e pigmentação das resinas compostas são dependentes da técnica e material.

The effect of shade and curing mode on cure efficiency of dual-cure resin cements / Efeito da cor e do modo de cura na eficiência de cura de cimentos resinosos de cura dual

Objective: To evaluate the effect of shade and curing mode on cure efficiency of two dual-cure resin cements. Material and Methods: Two shades (A2 and TRANS) of two different dual-cure resin cements (G-CEM, GC Dental and SET PP, SDI) were submitted to different curing modes: light curing through a 2 mm thick ceramic disc (IPS Empress Esthetic, A2, Ivoclar Vivadent) for 20 s (16 J/cm2), 40 s (32 J/ cm2) or 80 s (64 J/cm2) performed immediately or with 1 or 5 min of delay. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the degree of conversion (DC) after 48 h. For the statistical analysis, data were submitted to threeway analysis of variance, followed by Tukey’s test for multiple comparisons (p<0.05). Results: Shade influenced DC (%) for the dual-cure resin cements tested (p=0.00001). TRANS shade showed lower DC (%) for both cements when there was no delay before light-curing (p=0.00001). Curing mode also influenced DC (%) for the dual-cure resin cements tested: radiant exposure greater than 32 J/cm2 and delaying light-curing for 1 to 5 min increased the DC (%) for both dual-cure resin cements evaluated. Conclusions: One minute delay prior to light-curing improved the cure efficiency and may be a more clinically acceptable approach to increase the degree of conversion of dual-cure resin cements.

Objetivo: Avaliar a influência da cor e do modo de polimerização no grau de conversão de dois cimentos resinosos duais. Material e Métodos: Duas cores (A2 e TRANS) de dois cimentos resinosos duais (G-CEM, GC Dental e SET PP, SDI) foram submetidos a diferentes modos de cura: fotoativação através de um disco de cerâmica de 2 mm de espessura (IPS Empress Esthetic, A2, Ivoclar Vivadent) por 20 s (16 J/cm2), 40 s (32 J/cm2) ou 80 s (64 J/cm2) realizada imediatamente ou após 1 ou 5 min de espera. Espectrometria com transformada de Fourier (FT-IR) foi utilizada para avaliação do grau de conversão (GC) após 48 h. Para a análise estatística, os dados foram submetidos à análise de variância com três fatores, seguida de teste de Tukey para comparações múltiplas (p=0.05). Resultados: A cor influenciou o GC (%) dos cimentos resinosos duais avaliados (p=0.00001). A cor TRANS resultou menor GC (%) para ambos os cimentos quando não houve espera antes da fotoativação (p=0.00001). O modo de cura também teve influência n o GC (%) dos cimentos resinosos duais avaliados: doses de energia superiores a 32 J/cm2 e espera de 1 ou 5 min antes da fotoativação aumentou o GC (%) dos dois cimentos resinosos duais avaliados. Conclusão: Um min de espera antes da fotoativação melhorou a eficiência de cura e pode ser uma abordagem clinicamente aceitável para aumentar o grau de conversão de cimentos resinosos duais.

A clinical report of functional and esthetic oral rehabilitation in a high-caries-risk child: one-year follow-up / Caso clínico de reabilitação oral estética e funcional em criança de alto risco à cárie: um ano de acompanhamento

Este caso discutiu a abordagem multidisciplinar da reabilitação oral estética e funcional de uma criança com alto risco à cárie com perda prematurade dentes decíduos devido a complicações do tratamento endodôntico associado a cárie dentária generalizada. O paciente foi diagnosticado com mordida aberta anterior e deglutição atípica. O mesmo apresentou ansiedade e baixa auto-estima devido ao comprometimento estético. Combinação de procedimentos clínicos direcionados, com ênfase e foco no cuidado bucal domiciliar e o envolvimento ativo dos pais fez parte do processo de tratamento integral do paciente. Este foi examinado mensalmente por um odontopediatria, o qual orientou sobre hábitos alimentares, exposição a fluoretos, presença de biofilme, prevalência de cárie e má oclusão. Evidente melhora foi observada na função mastigatória,estética facial e comportamento psicológico aos 12 meses de acompanhamento clínico do caso.

This case discusses a multi-disciplinary approach to oral functional and esthetic rehabilitation on a high-caries-risk child with prematurely lost primary teeth due to endodontic complications associatedwith wide-spread dental caries. The patient was diagnosed with an anterior open bite and atypical swallowing. He exhibited anxiety and low selfesteemdue to esthetic impairment. A combination of targeted clinical procedures, a focus on oral home care and active parental involvement was integral to the treatment process. The patient was examined monthly by a pediatric dentist regarding dietary habits, fluoride exposure, biofilm= presence, caries prevalence, and malocclusion. Improvement was evident in patient’s masticatory function, facial esthetics, and psychological behavior at a 12-month follow-up evaluation.

Influence of chemical degradation and abrasion on surface properties of nanorestorative materials

Aim: The aim of this in vitro study was to investigate the synergistic effect of chemical degradation (erosion) and three-body abrasion (mechanical degradation) on the surface roughness (Ra) and hardness (KHN) of two nanorestorative materials and two conventional materials. Methods:Disc-shaped specimens (5 mm in diameter, 2 mm thick) of Filtek Z350TM and TPH SpectrumTM composites and Ketac NanoTM and VitremerTM light-curing glass ionomer cements, nanomaterials and conventional materials were prepared according to the manufacturer's instructions. After 24 h, polishing procedures were performed and initial measurements of Ra and KHN were taken in all specimens. The specimens were divided into 12 groups (n = 10) according to material and storage media: artificial saliva, orange juice, and Coca-Cola(r). After 30 days of storage, the specimens were submitted to mechanical degradation and re-evaluated for Ra and KHN. Data were tested for significant differences by repeated-measure three-way ANOVA and Tukey's tests (p<0.05). Results:Erosion and abrasion wear significantly decreased hardness of all materials. Only Filtek Z350 roughness, however, was not affected by erosion and abrasion. All materials showed a significant increase in surface roughness after erosion and abrasion, except for Filtek Z350. After chemical and mechanical degradation, the KHN of all samples had decreased significantly. After mechanical degradation, the acidic drinks (Coca-Cola(r) and orange juice) were more aggressive than artificial saliva to all materials. Conclusions:A synergistic effect was observed by the increase in roughness for all materials, except for Filtek Z350; hardness values decrease for all materials, regardless of whether they were nanofilled or not. The RMGICs were more susceptible to degradation than the composites, considering both hardness and roughness surface parameters.

Antimicrobial Activity of Two Root Canal Filling Pastes in Deciduous Teeth: an In Vitro Study / Avaliação "in vitro" da Atividade Antimicrobiana de Duas Pastas Obturadoras de Canais Radiculares de Dentes Decíduos

O tratamento endodôntico de dentes decíduos é de suma importância para que se possa preservar a dentição primária e, assim, promoverdesenvolvimento craniofacial adequado, oclusão normal e qualidades estéticas. No entanto, para que isso ocorra, é necessário o uso de pastasobturadoras com propriedades biológicas importantes para a preservação do dente. O objetivo deste estudo foi avaliar in vitro a atividadeantimicrobiana de duas pastas obturadoras utilizadas em dentes decíduos, Vitapex® e Calcipex®, em espécies microbianas comumenteencontradas em infecções endodônticas (Candida albicans, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichiacoli e Staphylococcus aureus), utilizando o teste de difusão em ágar. Foram utilizadas placas de BHI (Brain Heart Infusion) e confeccionadosquatro pontos equidistantes, imediatamente preenchidos com o Vitapex® cimentos e Calcipex®. O gluconato de clorexidina a 1% (CHX) e águadestilada foram utilizados como controle positivo e negativo, respectivamente. Após a incubação das placas a 37 °C durante 24 h, o diâmetrodas zonas de inibição do crescimento bacteriano ao redor dos poços foi medido (em milímetros) com um compasso de calibre digital com luzrefletida. Este teste foi realizado em triplicata e os dados foram submetidos à análise de variância e teste de Tukey (? = 0,05). Ambas as pastastiveram atividade antimicrobiana, mas Calcipex® foi mais eficiente para a maioria das cepas. O halo formado nos experimentos contendoas cepas de S. mutans e S. sanguinis foi semelhante para ambas as pastas. No presente estudo, podemos sugerir que Calcipex® apresentou amelhor propriedade antimicrobiana em relação aos microrganismos estudados.

Atuação interdisciplinar odontologia/fonoaudiologia no tratamento de paciente com cárie precoce da infância / Interdisciplinary approach between dentistry and speech-language pathology in treatment of children with early childhood caries

A integração interdisciplinar entre odontologia e fonoaudiologia pode proporcionar tratamento adequado das alterações dentárias e miofuncionais. Este relato de caso clínico apresenta o tratamento odontológico em criança com três anos de idade com cárie precoce da infância leve, com consequente perda dos incisivos centrais superiores devido a trauma, a reabilitação estética e funcional e tratamento fonoaudiológico. Os procedimentos clínicos odontológicos foram instrução de higiene bucal, aconselhamento dietético e realização das restaurações com resina composta devido ao acometimento por lesão cariosa dos dentes 64, 84, 85, 74, 75 (oclusal); e dentes 51, 61, 52 e 62 (face vestibular). Após um ano de preservação foi realizada a exodontia dos dentes 51 e 61 (com história de trauma anterior à primeira consulta), devido a reabsorção externa avançada. Em seguida, mantenedor de espaço estético-funcional removível foi colocado na região anterior superior. Avaliação fonoaudiológica foi realizada utilizando o protocolo Miofuncional Orofacial (MBGR), sendo verificadas as funções orofaciais, mobilidade e tônus muscular. Foram atribuídos escores para cada item avaliado no protocolo. Confirmou-se dificuldade de mobilidade dos lábios e língua com diminuição do tônus da bochecha e alterações na fala. A terapia fonoaudiológica foi estabelecida durante três meses com periodicidade semanal, havendo melhora em todos os aspectos alterados, confirmados pela adequação dos escores do Protocolo MBGR. No tratamento odontológico foram observados resultados clinicamente satisfatórios para a criança e responsáveis. Concluiu-se que o trabalho interdisciplinar entre a Odontologia e Fonoaudiologia proporcionou tratamento adequado para as condições bucais apresentadas pela criança, proporcionando saúde bucal e prognóstico favorável.

The integration of interdisciplinary dental and speech therapy can provide adequate treatment of dental and myofunctional alterations. In this case report, the dental treatment of three-year-old children with early childhood caries, premature loss of primary maxillary incisors due trauma, esthetic and functional rehabilitation and myofunctional therapy is presented. The dental care procedures included oral hygiene instructions, dietary recommendations and resin composite restorations of teeth 64, 84, 85, 74 and 75 (occlusal surface) and 51, 61, 52 and 62 (smooth surface) which were affected by dental caries. After one year of follow-up, teeth 51 and 61 were extracted (dental trauma history reported in first appointment), because the teeth presented extensive external reabsorption. Subsequently, esthetic and functional space maintainers were placed in the maxillary anterior region. Speech evaluation was performed using the MBGR protocol (orofacial myofunctional), in which the orofacial functions, mobility and muscular tone were analyzed. Scores were attributed to each item available in the protocol. Thus, the difficulty of lip and tongue movements were confirmed, in addition to reduced cheek tonus and alterations in speech. Myofunctional therapy was instituted for three months, once a week and this led to improvement in all aspects, with changes being confirmed by adequate scores obtained in application of the MBRG protocol. After dental treatment, clinical results satisfactory to both children and parents were observed. It was concluded that interdisciplinary approach involving dentistry and myofunctional therapy provided adequate treatment for oral conditions presented by children, providing oral health and favorable prognosis.

Reabilitação estética e funcional na primeira infância: relato de caso / Aesthetic and functional rehabilitation in infancy: a case report

A perda precoce dos dentes decíduos pode ocorrer pela falta de diagnóstico e tratamento na fase inicial da cárie dentária. O objetivo foi relatar o tratamento reabilitador estético e funcional de uma criança de 4 anos e 5 meses de idade, do gênero feminino, com cárie precoce da infância severa. Durante a anamnese, além da queixa de dor nos dentes, notou-se apatia da criança e constrangimento da mesma ao falar e sorrir. Ao exame clínico inicial, a paciente apresentou ausência dos dentes 55, 54, 75, 84 e 85; lesões de cárie ativa do 73 ao 83; lesões cariosas ativas extensas (53, 52, 51, 61, 62, 63, 64, 65, 74) e presença de fístula na região dos dentes 51 e 52. Após o exame radiográfico, o tratamento proposto foi a realização da exodontia dos elementos dentários não passíveis de receber tratamento restaurador (53, 52, 51, 61, 62, 63, 64, 65, 74); restauração com resina composta do 73 ao 83; e posterior colocação de prótese total superior e prótese parcial inferior. Orientações sobre higiene bucal e dieta não-cariogênica foram realizadas em todas as etapas do tratamento para incentivar a criança e familiares à prática de hábitos saudáveis. Diante do tratamento realizado, concluiu-se que a reabilitação estética-funcional recuperou as funções estéticas, fonéticas e mastigatórias da paciente; colaborando para a melhora da autoestima da criança o que gerou satisfação dos familiares. O trabalho educativo em estimular a criança e os cuidadores foi essencial para o sucesso do tratamento e manutenção da saúde bucal...

Dental caries is a disease related with sucrose rich diet and poor oral hygiene. During childhood, if caries disease is misdiagnosed, it can lead to early loss of primary teeth. This study aimed to report the aesthetic and functional rehabilitation treatment in a female child aged 4 years and 5 months, with severe early childhood caries. In the case history, as well as complaints of toothache, the child showed apathy and embarrassment to talk and smile. In initial clinical examination, it was noticed lack of teeth 55, 54, 85, 84 and 75; active caries lesion on teeth 71, 73, 81, 82 and 83; the dental elements 51, 52, 53, 61, 62, 63, 64, 65 and 74 presented with extensive coronary destruction bellow the gingival margins, and the teeth 51 and 52 with the presence of fistula. After radiographic evaluation, the treatment proposed was comprised by destroyed teeth extraction, resin composite restoration of all cavities and subsequent placement of upper denture and lower partial denture. Oral hygiene and non-cariogenic diet orientations were accomplished since the initial treatment to encourage the child and her family to practice healthy habits. After accomplished the treatment, it could be concluded that aesthetic and functional rehabilitation recovered the patient aesthetic, phonetic and mastication functions. This fact contributed to improve the child’s self-esteem and it promoted satisfaction of her family. The dentist educational work and the stimulus of child and her caregivers were essential for treatment successful and for patient oral health maintenance...

Dentin Bond Strength of a Fluoride-Releasing Adhesive System Submitted to pH-Cycling

To evaluate the microtensile bond strength (µTBS) of a fluoride-containing adhesive system submitted to a pH-cycling and storage time regimen for primary outcomes. As secondary outcomes the fluoride released amount was evaluated. Twelve dentin surfaces from sound third molar were divided into 2 groups according to adhesive systems: Clearfil SE Protect (PB) and Clearfil SE Bond (SE). Sticks obtained (1.0 mm2) from teeth were randomly divided into 3 subgroups according to storage regimen model: immediate (24h); 5-month deionized water (W); and pH-cycling model (C). All sticks were tested for µTBS in a universal testing machine. Fluoride concentration was obtained from 1-4 days and 30-day in W and 1-4 days in demineralization (DE)/remineralization (RE) solutions from C, using a fluoride-specific electrode. µTBS and fluoride released data were, respectively, submitted to ANOVA in a split plot design and Tukey, and Friedman' tests (a=0.05). There was no significant interaction between adhesive system and storage regimen for µTBS. W showed the lowest µTBS values. There was no significant difference between 24 h and C models for µTBS. There was no significant difference between adhesive systems. Failure mode was predominantly cohesive within composite for the 24 h and W, for the C group it was mixed for SE and cohesive within composite for PB adhesive system. Fluoride concentrations in the DE/RE solutions were less than 0.03125 ppm and not detected in W. In conclusion, the fluoride-containing adhesive system performed similarly to the regular one. Hydrolytic degradation is the main problem with both adhesive systems, regardless of fluoride contents.

O objetivo principal desse estudo foi avaliar a resistência de união à microtração de dois sistemas adesivos (com e sem flúor) após a ciclagem de pH e armazenagem em água deionizada. A quantidade de flúor liberada foi avaliada secundariamente. Doze terceiros molares hígidos foram separados em 2 grupos de acordo com o sistema adesivo: Clearfil SE Protect - com flúor (PB) e Clearfil SE Bond - sem flúor (SE). Os palitos (1 mm2) obtidos do mesmo dente foram aleatoriamente divididos em 3 subgrupos de acordo com o meio de armazenagem: em água deionizada por 24h ou 5 meses e ciclagem de pH. Os palitos foram tracionados em uma máquina de ensaio universal a 0,5 mm/min. A concentração de flúor foi analisada em água deionizada (1-4 dias e 30 dias) e na solução remineralizadora e desmineralizadora (1-4 dias) usando um eletrodo específico. Os dados de resistência de união e liberação de flúor foram, respectivamente, submetidos à Análise de Variância em esquema de parcela subdividida e ao teste de Friedman (a=0,05). Não houve nenhuma interação significativa na resistência de união entre os sistemas adesivos e os meios de armazenagem. Os menores valores de resistência de união à microtração foram encontrados para os palitos armazenados em água deionizada. Não houve nenhuma diferença significativa nos valores de resistência de união após 24h e ciclagem de pH. Nenhuma diferença significativa na resistência de união foi observada entre os 2 sistemas adesivos. O modo de falha foi predominantemente coesivo em compósito para os grupos armazenados em água por 24h ou 5 meses para ambos os sistemas adesivos. No grupo submetido à ciclagem, a falha foi mista para o SE e coesiva em compósito para o PB. A concentração de flúor nas soluções DE/RE foi menor que 0,03125 ppm e não detectada em água deionizada. Concluindo, o sistema adesivo com flúor (PB) apresentou performance similar ao sistema adesivo sem flúor (SE). A degradação hidrolítica foi o principal fator para ambos os sistemas adesivos, independente da adição de flúor.